based methylation status (Pyrosequencing Inc)
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Based Methylation Status, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/based methylation status/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
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1) Product Images from "MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR"
Article Title: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR
Journal: Journal of Translational Medicine
doi: 10.1186/1479-5876-10-36
Figure Legend Snippet: Primers and probes used for quantitative methylation-specific polymerase chain reaction (qMSP)
Techniques Used: Methylation, Polymerase Chain Reaction, Sequencing
Figure Legend Snippet: MGMT methylation frequencies and methylation level in methylated glioma samples
Techniques Used: Methylation, CpG Methylation Assay
Figure Legend Snippet: Overall survival in primary glioblastoma patients treated with standard radiotherapy and concomitant temozolomide . ( A ) Methylation status based on results from qMSP. Blue line; methylated, red line; unmethylated. ( B ) Methylation status based on results from pyrosequencing. Blue line; methylated, red line; unmethylated. ( C ) Methylation status based on both qMSP and pyrosequencing results. Blue line; methylated in both methods, red line; unmethylated in both methods, green line; methylated in pyrosequencing assay and unmethylated in qMSP assay. Abbreviation: qMSP, quantitative methylation specific PCR
Techniques Used: Methylation, Pyrosequencing Assay
Figure Legend Snippet: Patient characteristics and results of survival analysis for primary glioblastoma patients when stratified by MGMT methylation status
Techniques Used: Methylation, Standard Deviation
Figure Legend Snippet: Gene expression level of MGMT is associated with promoter DNA methylation status . MGMT gene expression in methylated (blue box plots) and unmethylated (red box plots) tissue samples analyzed by two different primer/probe sets (Assay 1; Hs00172470_m1 and assay 2; Hs01037698_m1). ( A ) Methylation status based on results from qMSP. ( B ) Methylation status based on results from pyrosequencing. Abbreviation: qMSP, quantitative methylation specific PCR
Techniques Used: Gene Expression, DNA Methylation Assay, Methylation
Figure Legend Snippet: Scatter plots of MGMT gene expression quantity in methylated and unmethylated samples . Red color indicates samples with negative methylation status by qMSP and pyrosequencing, blue color indicates samples with positive methylation status by qMSP and pyrosequencing, and green color indicate samples with non-concordant methylation status in the qMSP and pyrosequencing analysis. Circles indicate samples with loss of 10q26, whereas dots represent samples without loss of this region. Plots are based on the normalized gene expression detected by primer/probe set Hs00172470_m1. The plots based on the normalized gene expression detected by the other primer/probe set, Hs01037698_m1, were similar (data not shown). Abbreviation: qMSP, quantitative methylation specific PCR
Techniques Used: Gene Expression, Methylation